coenzyme A + 4-coumarate + ATP → (E)-4-coumaroyl-CoA + diphosphate + AMP (R2776)
- Overexpression
  - Plasmid
    - Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT
L-tyrosine → 4-coumarate + ammonium (R3408)
- Overexpression
  - Plasmid
    - Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT
S-adenosyl-L-methionine + 4-hydroxybenzoate → S-adenosyl-L-homocysteine + methylparaben (R11696)
- Overexpression
  - Plasmid
    - Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT
(E)-4-coumaroyl-CoA + H₂O → acetyl-CoA + 4-hydroxybenzaldehyde (R00894)
- Overexpression
  - Plasmid
    - Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT
H₂O + 4-hydroxybenzaldehyde + NAD⁺ → H⁺ + NADH + 4-hydroxybenzoate (R2095)
- Overexpression
  - Plasmid
    - Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT