Escherichia coli BL21(DE3)pLysS/pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pLysS added | Plasmid pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT addedBars (|) indicate differences between strains.
Plasmids
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pACE1-PAL/TAL-4CL-HCHL-VDH-BSMT (PAL/TAL, 4CL, HCHL, VDH and BSMT)
pLysS (T7 lysozyme)
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| methylparaben | Titer | 3.0 µg/L | 24 h | Flask | [362] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.