• (2E,6E)-farnesyl diphosphate → presqualene diphosphate + diphosphate (R1186)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D
• NADP⁺ + squalene + diphosphate ↔ NADPH + presqualene diphosphate + H⁺ (R1185)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D
• isopentenyl diphosphate + prenyl diphosphate ↔ diphosphate + geranyl diphosphate (R315)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D
• isopentenyl diphosphate + geranyl diphosphate ↔ diphosphate + (2E,6E)-farnesyl diphosphate (R574)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D
• isopentenyl diphosphate ↔ prenyl diphosphate (R123)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
• pyruvate + H⁺ + D-glyceraldehyde 3-phosphate → 1-deoxy-D-xylulose 5-phosphate + CO₂ (R270)
  • Overexpression
    • Plasmid
      • Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS