Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
Ancestors
Genotype with respect to wild type
F− dcm gal ompT hsdS(rB- mB-) | F− ompT hsdSB (rB−mB−) gal dcm λ(DE3) | Plasmid pIDXS added | Plasmid pHOP-D added | Plasmid pHOP-AB addedBars (|) indicate differences between strains.
Plasmids
Modifications
| Origin | Gene | Organism | Modification |
|---|---|---|---|
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Overexpression
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Production
| Metabolites | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|
| squalene | Titer | 11.8 mg/L | Flask | [53] |
* Inferred from plots using RetroPlot.
Heterologous.
Target metabolites are shown in bold, while non-bold metabolites represent intermediates or potential byproducts.
References
- Ghimire, Gopal Prasad; Lee, Hei Chan & Sohng, Jae Kyung (2009). Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli. Applied and Environmental Microbiology, 75(22), 7291-7293.