Ghimire, Gopal Prasad; Lee, Hei Chan & Sohng, Jae Kyung (2009). Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli. Applied and Environmental Microbiology, 75(22), 7291-7293.

Strains

• Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS
• Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D

Plasmids

• pGEM-T Easy vector
• pET-28a(+)
• pETDuet-1
• pCDFDuet-1
• pHOP-A
• pHOP-B
• pHOP-AB
• pHOP-D
• pIDXS

Metabolite production

• squalene
  Titer
  • 11.8 mg/L (Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D/pIDXS in Flask)
  • 4.1 mg/L (Escherichia coli BL21(DE3)/pHOP-AB/pHOP-D in Flask)