pPK10

Ancestors

Main features

pBR322 ori, P_trc-synRBS- pgk- synRBS- serA^Δ197- synRBS- serB- synRBS- serC

Selection marker:

Ampicillin

Strains harboring plasmid pPK10

Escherichia coli E3 (pPK10)

Escherichia coli E4G1 (pPK10)

Escherichia coli E4G2 (pPK10)

References

Zhang, Y.; Kang, P.; Liu, S.; Zhao, Y.; Wang, Z.; Chen, T. (2017). GlyA Gene Knock-out in Escherichia coli Enhances L-Serine Production without Glycine Addition. Biotechnol. Bioprocess Eng. 22, 390–396.