pTrcA*H

Ancestors

All plasmids

pTrc99A

pTrcA*H

Main features

P_trc-metA^fbr(A881G)-yjeH

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*metA*	Escherichia coli	Feedback insensitive
*yjeH*	Escherichia coli	Overexpression

Strains harboring plasmid pTrcA*H

Escherichia coli W3110 ΔmetJ (pTrcA*H)

Escherichia coli W3110 ΔmetJΔmetI (pTrcA*H)

Escherichia coli W3110 ΔmetJΔmetIΔlysA/pTrcA*H

Escherichia coli W3110 ΔmetJΔmetIΔlysAΔthrB/pTrcA*H

Escherichia coli W3110 ΔmetJΔmetIΔthrB/pTrcA*H

References

Jian‐Feng Huang, Zhi‐Qiang Liu, Li‐Qun Jin, Xiao‐Ling Tang, Zhen‐Yang Shen, Huan‐Huan Yin & Yu‐Guo Zheng (2016). Metabolic engineering of Escherichia coli for microbial production of L‐methionine. Biotechnology & Bioengineering.