pCS-trpE^fbrG-APTA

Ancestors

All plasmids

pCS27

pCS-trpE^fbrG

pCS-trpE^fbrG-APTA

Main features

trpE^fbr(S40R) trpG(N-terminal domain of trpD) aroL ppsA tktA aroG^fbr(D146N)

Selection marker:

Kanamycin

Modifications

Gene	Organism	Modification
*trpE*	Escherichia coli	Feedback insensitive
*trpD*	Escherichia coli	Overexpression
*aroL*	Escherichia coli	Overexpression
*ppsA*	Escherichia coli	Overexpression
*tktA*	Escherichia coli	Overexpression
*aroG*	Escherichia coli	Feedback insensitive

Strains harboring plasmid pCS-trpE^fbrG-APTA

Escherichia coli MA5

Escherichia coli MA4

References

Jian Wang, Ruihua Zhang, Yan Zhang, Yaping Yang, Yuheng Lin & Yajun Yan (2019). Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production. Metabolic Engineering.