pCTSDT

Ancestors

All plasmids

pETDuet-1

pCTSDT

Derivated

Main features

P_T7-ilvC_Ec-T_T7-P_T7-alsS_Bs-P_T7-ilvD_Ec-T_T7

Selection marker:

Ampicillin

Modifications

Gene	Organism	Modification
*ilvC*	Escherichia coli	Overexpression
*alsS*	Bacillus subtilis	Overexpression
*ilvD*	Escherichia coli	Overexpression

Strains harboring plasmid pCTSDT

Escherichia coli 050T4/pCTSDT

Escherichia coli B0016-050/pCTSDT

Escherichia coli 050T/pCTSDT

Escherichia coli BL21(DE3)/pCTSDT

Escherichia coli 050T3/pCTSDT

Escherichia coli 050T1/pCTSDT

Escherichia coli 050T2/pCTSDT

Escherichia coli 050T3/pCTSDT+pACYC-pntAB

Escherichia coli 050T4-1/pCTSDT

Escherichia coli 050T4-2/pCTSDT

References

Li Zhou, Ying Zhu, Zhongzhe Yuan, Guangqing Liu, Zijin Sun, Shiyu Du, He Liu, Yating Li, Haili Liu, and Zhemin Zhou (2022). Evaluation of Metabolic Engineering Strategies on 2-Ketoisovalerate Production by Escherichia coli. Applied and Environmental Microbiology. 88(17): e00976-22.