pTrc99A
Ancestors
Derivated
Main features
Ptrc, ColE1 oriSelection marker:
Ampicillin
Strains harboring plasmid pTrc99A
Escherichia coli DH5α/pTrc99A, pLyc184
References
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Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.
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Mi Tang, Xuewei Pan, Tianjin Yang, Jiajia You, Rongshuai Zhu, Taowei Yang, Xian Zhang, Meijuan Xu, Zhiming Rao (2023). Multidimensional engineering of Escherichia coli for efficient synthesis of L-tryptophan. Bioresource Technology. 386.
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Yu Deng, Ning Ma, Kangjia Zhu, Yin Mao, Xuetuan Wei & Yunying Zhao (2018). Balancing the carbon flux distributions between the TCA cycle and glyoxylate shunt to produce glycolate at high yield and titer in Escherichia coli. Metabolic Engineering.
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Han Liu, Yehua Hou, Yu Wang & Zhimin Li (2019). Enhancement of Sulfur Conversion Rate in the Production of l‑Cysteine by Engineered Escherichia coli. Journal of Agricultural & Food Chemistry.
José Luis Báez‐Viveros, Joel Osuna, Georgina Hernández‐Chávez, Xavier Soberón, Francisco Bolívar & Guillermo Gosset (2004). Metabolic engineering and protein directed evolution increase the yield of L‐phenylalanine synthesized from glucose in Escherichia coli. Biotechnology & Bioengineering.
Vo, Toan Minh & Park, Sunghoon. Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose. Metabolic Engineering. 2022, 73, 104-113.
Pu, W., Chen, J., Zhou, Y. et al. Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid. Biotechnol Biofuels 16, 31 (2023).
Jiang, Youming; Zheng, Tianwen; Ye, Xiaohan; Xin, Fengxue; Zhang, Wenming; Dong, Weiliang; Ma, Jiangfeng & Jiang, Min. Metabolic engineering of Escherichia coli for l-malate production anaerobically. Microbial Cell Factories. 2020, 19(1).