pZPW85

Ancestors

Main features
hemAC75A/R365K, PJ23100-coaA, Pppc-ppc, PeamA-eamA, PgrxA-grxA, PkatG-katG

Selection marker:

Ampicillin



Modifications
Gene Organism Modification
Feedback insensitive
Overexpression
ppc
Overexpression
Overexpression
Overexpression
Overexpression



Strains harboring plasmid pZPW85

Escherichia coli ALA23


References

Pu, W., Chen, J., Zhou, Y. et al. Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid. Biotechnol Biofuels 16, 31 (2023).