pACYC177

Ancestors

Derivated

pSV

pACYCppc

pACYCaceBA

pACYC-yhaM1

Main features

p15A ori

Selection marker:

Kanamycin

Ampicillin

Strains harboring plasmid pACYC177

Escherichia coli TH07 (pBRThrABC, pACYC177)

Escherichia coli TH09C (pBRThrABC, pACYC177)

Escherichia coli TH11C (pBRThrABC, pACYC177)

Escherichia coli MG1655 (pMIV-cysE5, pACYC177)

References

Zhi-Jun Zhao, Chun Zou, Yi-Xing Zhu, Jun Dai, Sheng Chen, Dan Wu, Jing Wu & Jian Chen (2011). Development of l-tryptophan production strains by defined genetic modification in Escherichia coli. Journal of Industrial Microbiology & Biotechnology.

Kwang Ho Lee, Jin Hwan Park, Tae Yong Kim, Hyun Uk Kim & Sang Yup Lee (2007). Systems metabolic engineering of Escherichia coli for L‐threonine production. Molecular Systems Biology.

Gen Nonaka & Kazuhiro Takumi (2017). Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli. AMB Express.