Titer in Flask for (-)-patchoulol

Strain Production
mg/L
80.0
160.0
240.0
320.0
400.0
E. coli PTS1
E. coli PTS2
E. coli 010H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 040H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 050H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)

Titer in Fed-Batch for (-)-patchoulol

Strain Production
mg/L
200
400
600
800
1000
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)

Biomass-specific yield in Flask for (-)-patchoulol

Strain Production
mg/g DCW
10
20
30
40
50
E. coli 010H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 040H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 050H (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)

Volumetric productivity in Flask for (-)-patchoulol

Strain Production
mg/L/h
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)

Volumetric productivity in Fed-Batch for (-)-patchoulol

Strain Production
mg/L/h
E. coli 060HA (pMevΔFPPS/pET-FPPS-(PT)4P-PTS2C415F/H454A)