Escherichia coli ALA13

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli MG1655

Escherichia coli ALA13

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pZPW74 added | Plasmid pZCA136 added

Bars (|) indicate differences between strains.

Plasmids

pZCA136 (sRsucC, sRhemB, sRaceA, P_tet, T1/TE)

pZPW74 (hemA^C75A/R365K, P_J23100-coaA, P_ppc-ppc, P_eamA-eamA)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
5-aminolevulinate		Titer	5.69 g/L	6.739 OD₆₀₀^*	Glucose		Flask	[ 239 ]
5-aminolevulinate		Substrate yield	0.495 mol/mol of substrate	6.739 OD₆₀₀^*	Glucose		Flask	[ 239 ]
5-aminolevulinate		Titer	6.857 g/L ^*	7.674 OD₆₀₀^*	Glucose		Flask	[ 239 ]
5-aminolevulinate		Substrate yield	0.45 mol/mol of substrate ^*	7.674 OD₆₀₀^*	Glucose		Flask	[ 239 ]
5-aminolevulinate		Titer	8.008 g/L ^*	6.049 OD₆₀₀^*	Glucose		Flask	[ 239 ]

* Inferred from plots using RetroPlot.

References

Pu, W., Chen, J., Zhou, Y. et al. Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid. Biotechnol Biofuels 16, 31 (2023).