Escherichia coli S028

Ancestors

Derived strains

Genotype with respect to parental

P_trc -trpE^fbrDCBA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
trpE	Escherichia coli	Feedback insensitive
trpD	Escherichia coli	Overexpression
trpC	Escherichia coli	Overexpression
trpB	Escherichia coli	Overexpression
trpA	Escherichia coli	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	19.0 g/L		Glucose	42 h	Fed-Batch	[ 165 ]
L-tryptophan		Substrate yield	0.15 g/g of sustrate		Glucose	42 h	Fed-Batch	[ 165 ]

* Inferred from plots using RetroPlot.

References

Lin Chen & An-Ping Zeng (2016). Rational design and metabolic analysis of Escherichia coli for effective production of L-tryptophan at high concentration. Applied Microbiology and Biotechnology.

Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.