Escherichia coli ALA26
Ancestors
Genotype with respect to parental
ΔcoaA
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pZPW76 added | Plasmid pZCA136 added | ΔcoaABars (|) indicate differences between strains.
Plasmids
-
pZCA136 (sRsucC, sRhemB, sRaceA, Ptet, T1/TE)
pZPW76 (hemAC75A/R365K, PJ23100-coaA, Pppc-ppc, PeamA-eamA, PgrxA-grxA)
Modifications
Gene | Organism | Modification |
---|---|---|
Silencing
|
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
5-aminolevulinate | Titer | 30.7 g/L | 31.9 OD600* | Glucose | 32 h * | Fed-Batch | [ 239 ] | |
5-aminolevulinate | Substrate yield | 0.532 mol/mol of substrate | 31.9 OD600* | Glucose | 32 h * | Fed-Batch | [ 239 ] | |
5-aminolevulinate | Volumetric productivity | 1.02 g/L/h | 31.9 OD600* | Glucose | 32 h * | Fed-Batch | [ 239 ] |
* Inferred from plots using RetroPlot.