Escherichia coli S092/pAnTrpC

Ancestors

Genotype with respect to parental

trpC(A. niger)

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA | ΔtrpC | Plasmid pAnTrpC added | trpC(A. niger)

Bars (|) indicate differences between strains.

Plasmids

pAnTrpC (pSC101 ori, P_tac::trpC_An)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Substrate yield	0.11 g/g of sustrate		Glucose	20 h	Batch	[ 165 ]
L-tryptophan		Titer	3.22 g/L		Glucose	20 h	Batch	[ 165 ]

* Inferred from plots using RetroPlot.

References

Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.