Escherichia coli S092/(pZATrpE40+pEcTrpC)

Ancestors

Genotype with respect to parental

pTac::trpC P_LtetO−1::trpE^fbr

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacU169 gal490 λcI857 Δ(cro-bioA) | rpsL (StrR) | ∆aroF ∆aroG ∆mtr ∆tnaA ∆tnaB ∆λ | ∆aroH::P_{J23119-rpsL-tac} -(aroG^fbr-serA^fbr) | P_trc -trpE^fbrDCBA | ΔtrpC | Plasmid pEcTrpC added | Plasmid pZATrpE40 added | pTac::trpC P_LtetO−1::trpE^fbr

Bars (|) indicate differences between strains.

Plasmids

pZATrpE40 (p15A ori, P_LtetO−1::trpE^fbr)

pEcTrpC (pSC101 ori, P_tac, trpC)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Substrate yield	0.14 g/g of sustrate		Glucose		Fed-Batch	[ 165 ]

* Inferred from plots using RetroPlot.

References

Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.