Escherichia coli NT1259

Ancestors

Derived strains

Escherichia coli NT1259 shiA_Cg

Genotype with respect to parental

ΔtrpL trpE^FBR –(M293 T N168D A478 T C237R) ΔtrpR::FRT ΔtnaA::FRT ΔsdaB::FRT Δlac:: P_tac-aroF-B-L Δfuc::P_tac-tktA Δxyl::P_tac-serA^FBR (T372 N) Δrib::P_tac-trpB-trpA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔtrpL trpE^FBR –(M293 T N168D A478 T C237R) ΔtrpR::FRT ΔtnaA::FRT ΔsdaB::FRT Δlac:: P_tac-aroF-B-L Δfuc::P_tac-tktA Δxyl::P_tac-serA^FBR (T372 N) Δrib::P_tac-trpB-trpA

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
trpL	Escherichia coli	Silencing
trpE	Escherichia coli	Feedback insensitive
trpR	Escherichia coli	Silencing
tnaA	Escherichia coli	Silencing
sdaB	Escherichia coli	Silencing
aroF	Escherichia coli	Overexpression
aroB	Escherichia coli	Overexpression
aroL	Escherichia coli	Overexpression
tktA	Escherichia coli	Overexpression
serA	Escherichia coli	Feedback insensitive
trpB	Escherichia coli	Overexpression
trpA	Escherichia coli	Overexpression

References

Julia Tröndle, Kristin Schoppel, Arne Bleidt, Natalia Trachtmann, Georg A. Sprenger, Dirk Weuster-Botz (2020). Metabolic control analysis of L-tryptophan production with Escherichia coli based on data from short-term perturbation experiments. Journal of Biotechnology, 307, 15-28.

Kristin Schoppel, Natalia Trachtmann, Emil J. Korzin, Angelina Tzanavari, Georg A. Sprenger & Dirk Weuster-Botz (2022). Metabolic control analysis enables rational improvement of E. colil-tryptophan producers but methylglyoxal formation limits glycerol-based production. Microbial Cell Factories.