Escherichia coli Q1 (DE3)

Ancestors

Derived strains

• Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
• Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
• Escherichia coli Q1 (DE3) ThrA Y356C
• Escherichia coli Q1(DE3) ThrA S359R
• Escherichia coli Q1 (DE3) ThrA S357R

Genotype with respect to parental

λ(DE3)

Genotype with respect to wild type

F⁺ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | λ(DE3)

Bars (|) indicate differences between strains.

References

• Hemanshu Mundhada, Konstantin Schneider, Hanne Bjerre Christensen & Alex Toftgaard Nielsen (2015). Engineering of high yield production of L‐serine in Escherichia coli. Biotechnology & Bioengineering.

• Hemanshu Mundhada, Jose M. Seoane, Konstantin Schneider, Anna Koza, Hanne B. Christensen, Tobias Klein, Patrick V. Phaneuf, Markus Herrgard, Adam M. Feist & Alex T. Nielsen (2016). Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution. Metabolic Engineering.