Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)

Ancestors

Genotype with respect to parental

λ(DE3)


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | λ(DE3) | Plasmid pACTCDuet-1-serB added | Plasmid pCDF-Duet-serAmut-serC added | λ(DE3)

Bars (|) indicate differences between strains.


Plasmids

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
L-serine Titer 0.89 g/L Glucose Flask [ 144 ]
L-serine Volumetric productivity 2.73 g/L/h Glucose Flask [ 144 ]
L-serine Titer 8.3 g/L Glucose Fed-Batch [ 144 ]
L-serine Volumetric productivity 0.18 g/L/h Glucose Fed-Batch [ 144 ]
L-serine Substrate yield 0.12 g/g of sustrate Glucose Fed-Batch [ 144 ]
L-serine Titer 3.7 g/L Glucose 52 h Fed-Batch [ 192 ]

* Inferred from plots using RetroPlot.


References