Escherichia coli Q3 (DE3) (pCDF-Duet-serAmut-serC, pACYCDuet-1-serB-eamA-c-His)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | ΔsdaA | ΔsdaB ΔtdcG | ΔglyA | ΔrhtA ΔompX ΔopgE ΔrybA ΔmntS | λ(DE3) | Plasmid pACYCDuet-1-serB-eamA-c-His added | Plasmid pCDF-Duet-serAmut-serC addedBars (|) indicate differences between strains.
Plasmids
-
pCDF-Duet-serAmut-serC (serAmut serC)
pACYCDuet-1-serB-eamA-c-His (serB eamA)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
L-serine | Titer | 11.73 g/L | Glucose | Fed-Batch | [ 144 ] | |||
L-serine | Volumetric productivity | 0.24 g/L/h | Glucose | Fed-Batch | [ 144 ] | |||
L-serine | Substrate yield | 0.15 g/g of sustrate | Glucose | Fed-Batch | [ 144 ] |
* Inferred from plots using RetroPlot.