Escherichia coli TS-2

Ancestors

All strains

Escherichia coli K12

Escherichia coli W1485

Escherichia coli W2637

Escherichia coli W3110

Escherichia coli KW

Escherichia coli KW001

Escherichia coli TS-2

Genotype with respect to parental

ΔglnA::BMglnA

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | Plasmid PH5a1 added | ΔglnA::BMglnA

Bars (|) indicate differences between strains.

Plasmids

PH5a1 (aroG^fbr trpEDCBA)

Modifications

Gene	Organism	Modification
glnA	Escherichia coli	Silencing
glnA	Bacillus megaterium	Overexpression

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-tryptophan		Titer	0.62 g/L		Glucose	48 h	Flask	[ 136 ]

* Inferred from plots using RetroPlot.

References

Zhu Li, Dongqin Ding, Huiying Wang, Linxia Liu, Huan Fang, Tao Chen & Dawei Zhang (2020). Engineering Escherichia coli to improve tryptophan production via genetic manipulation of precursor and cofactor pathways. Synthetic and Systems Biotechnology.