Escherichia coli UR6

Ancestors

Genotype with respect to parental

△nupC, △nupG


Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | △lacI, yghX::Ptrc-pyrB-pyrC-pyrAA-pyrABfbr(E949*)-pyrK-pyrD-pyrF-pyrE | Δudk | Δudp | △rihA | △rihB | △rihC | tyrR::Ptrc-pyrAA-pyrAB | △argF | Pppc::Ptrc, △thrA, △iclR | △purR | trpR::prs | △nupC, △nupG

Bars (|) indicate differences between strains.


Modifications
Gene Organism Modification
Silencing
Silencing

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
uridine Titer 14.5 g/L 33.6 OD600 Glucose 32 h Flask [ 202 ]
uridine Substrate yield 0.161 g/g of sustrate 33.6 OD600 Glucose 32 h Flask [ 202 ]
uracil Titer 0.52 g/L 33.6 OD600 Glucose 32 h Flask [ 202 ]
uridine Titer 70.3 g/L 68.0 OD600 Glucose 64 h Fed-Batch [ 202 ]
uridine Substrate yield 0.259 g/g of sustrate 68.0 OD600 Glucose 64 h Fed-Batch [ 202 ]
uridine Volumetric productivity 1.1 g/L/h 68.0 OD600 Glucose 64 h Fed-Batch [ 202 ]
uracil Titer 0.9 g/L 68.0 OD600 Glucose 64 h Fed-Batch [ 202 ]

* Inferred from plots using RetroPlot.


References