Escherichia coli DH5α/pTrcDx-idi-fbaA, pLyc184
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTrcDx-idi-fbaA added | Plasmid pLyc184 addedBars (|) indicate differences between strains.
Plasmids
-
pLyc184 (crtEIB under gntT104 promoter)
pTrcDx-idi-fbaA (Ptrc dxs idi fbaA)
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| all-trans-lycopene |
|
Titer | 11.5 mg/L | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
| all-trans-lycopene |
|
Biomass-specific yield | 7.67 mg/g DCW | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
| all-trans-lycopene |
|
Specific production rate | 191.75 µg/g DCW/h | Yeast extract Tryptone | 48 h | Flask | [ 155 ] |
* Inferred from plots using RetroPlot.