Escherichia coli DH5α/pTrcDx-idi-mdh, pLyc184
Ancestors
Genotype with respect to wild type
F−, endA1, hsdR17 (r K −, m K + ), supE44, thi-l, λ−, recA1, gyrA96, ΔlacU169 (Φ80lacZ ΔM15) | Plasmid pTrcDx-idi-mdh added | Plasmid pLyc184 addedBars (|) indicate differences between strains.
Plasmids
-
pLyc184 (crtEIB under gntT104 promoter)
pTrcDx-idi-mdh (Ptrc dxs idi mdh)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
all-trans-lycopene |
|
Titer | 13.2 mg/L | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
all-trans-lycopene |
|
Biomass-specific yield | 9.98 mg/g DCW | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
all-trans-lycopene |
|
Specific production rate | 249.5 µg/g DCW/h | Yeast extract Tryptone | 48 h | Flask | [ 155 ] |
* Inferred from plots using RetroPlot.