Escherichia coli WLGB-RP/pTrcDx-idi-mdh, pLyc184
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | ΔgdhA | ΔgpmB | pTrc-ispA-dxs pTrc-idi | Plasmid pTrcDx-idi-mdh added | Plasmid pLyc184 addedBars (|) indicate differences between strains.
Plasmids
-
pLyc184 (crtEIB under gntT104 promoter)
pTrcDx-idi-mdh (Ptrc dxs idi mdh)
Production
| Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
|---|---|---|---|---|---|---|---|---|
| all-trans-lycopene |
|
Titer | 23.97 mg/L | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
| all-trans-lycopene |
|
Biomass-specific yield | 12.32 mg/g DCW | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
| all-trans-lycopene |
|
Specific production rate | 256.63 µg/g DCW/h | Yeast extract Tryptone | 48 h | Flask | [ 155 ] | |
| all-trans-lycopene |
|
Titer | 206.7 mg/L | Glucose | 36 h | Fed-Batch | [ 155 ] |
* Inferred from plots using RetroPlot.