Escherichia coli PM-14

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21(DE3)

Escherichia coli PM-14

Derived strains

Escherichia coli M1

Genotype with respect to wild type

F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pET-28aA added

Bars (|) indicate differences between strains.

Plasmids

pET-28aA (kan^R-A (rare codon usage))

Strain generated through random mutagenesis

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-proline		Titer	0.816 g/L		Glucose	24 h	Flask	[ 212 ]

* Inferred from plots using RetroPlot.

References

Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.