Escherichia coli M4

Ancestors

All strains

Escherichia coli B

Escherichia coli BL21(DE3)

Escherichia coli PM-14

Escherichia coli M1

Escherichia coli M2

Escherichia coli M3

Escherichia coli M4

Derived strains

Escherichia coli M5

Genotype with respect to parental

ΔproP

Genotype with respect to wild type

F⁻ ompT hsdS_B (r_B⁻m_B⁻) gal dcm λ(DE3) | Plasmid pET-28aA added | Plasmid pET-28aA lost | ΔputA | ΔputP | ΔproP

Bars (|) indicate differences between strains.

Modifications

Gene	Organism	Modification
proP	Escherichia coli	Silencing

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-proline		Titer	31.4 g/L		Glucose		Fed-Batch	[ 212 ]
L-proline		Volumetric productivity	0.785 g/L/h		Glucose		Fed-Batch	[ 212 ]

* Inferred from plots using RetroPlot.

References

Long M, Xu M, Ma Z, Pan X, You J, Hu M, Shao Y, Yang T, Zhang X, Rao Z. Significantly enhancing production of trans-4-hydroxy-l-proline by integrated system engineering in Escherichia coli. Sci Adv. 2020 May 22;6(21):eaba2383.