Escherichia coli EYX-2
Ancestors
Derived strains
Genotype with respect to wild type
F+ (λ) | IacIq rrnBT14 ∆lacZWJ16 hsdR514 ∆araBADAH33 ∆rhaBADLD78 | ΔaceB | ΔglcB::WAK cassette (ycdW, aceA-aceK)Bars (|) indicate differences between strains.
Strain generated through random mutagenesis
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
glycolate | Substrate yield | 0.48 g/g of sustrate | Glucose | Flask | [ 214 ] | |||
glycolate | Titer | 56.44 g/L | 19.26 OD600 | Glucose | 120 h | Fed-Batch | [ 225 ] | |
glycolate | Substrate yield | 0.52 g/g of sustrate | 19.26 OD600 | Glucose | 120 h | Fed-Batch | [ 225 ] | |
glycolate | Substrate yield | 0.33 g/g of sustrate | 2.11 OD600* | Glucose | Flask | [ 225 ] | ||
glycolate | Substrate yield | 0.48 g/g of sustrate | Glucose | 24 h | Batch | [ 225 ] | ||
glycolate | Titer | 2.66 g/L | 2.11 OD600* | Glucose | Flask | [ 225 ] | ||
glycolate | Titer | 8.96 g/L | Glucose | 24 h | Batch | [ 225 ] |
* Inferred from plots using RetroPlot.