Escherichia coli MG1655 (pMIV-cysE5, pACYC177)
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pMIV-cysE5 added | Plasmid pACYC177 addedBars (|) indicate differences between strains.
Plasmids
-
pACYC177 (p15A ori)
pMIV-cysE5 (p15A ori, PompC-cysE5)
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
L-cysteine | Titer | 74.6 mg/L | 21.7 OD600 | Glucose | 24 h | Flask | [ 226 ] | |
L-cysteine | Substrate yield | 0.187 % | 21.7 OD600 | Glucose | 24 h | Flask | [ 226 ] |
* Inferred from plots using RetroPlot.