Escherichia coli ILE01 (pTacilvAIH, pBRthrABCygaZH)

Ancestors

Genotype with respect to wild type

F⁺ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | ΔlacI | thrA^fbr | lysC^fbr | P_thr::P_tac | ΔlysA | ΔmetA | ilvA^C290T | Δtdh | iclR::Cm^R | P_ppc::P_trc | ΔCm^R | ilvA^{C1339T, G1341T, C1351G, T1352C} | Plasmid pBRthrABCygaZH added | Plasmid pTacilvAIH added

Bars (|) indicate differences between strains.

Plasmids

pTacilvAIH (ilvAIH)

pBRthrABCygaZH (thrA^fbrBC ygaZH)

Production

Metabolites	Heterologous	Production type	Production	Biomass	Carbon source	Time	Scale	Ref.
L-isoleucine		Titer	1.11 g/L		Glucose		Flask	[ 228 ]

* Inferred from plots using RetroPlot.

References

Jin Hwan Park, Jae Eun Oh, Kwang Ho Lee, Ji Young Kim & Sang Yup Lee (2012). Rational Design of Escherichia coli for l‑Isoleucine Production. ACS Synthetic Biology.