Escherichia coli ita23

Ancestors

Genotype with respect to parental

∆Picd::cam_P2 (BBa_J23115)


Genotype with respect to wild type
F+ (λ) | λ- | F- λ- ilvG- rfb-50 rph-1 | Plasmid pCadCs added | ΔaceA | ∆sucCD | ∆pykA ∆pykF::cam | ∆pta | ∆Picd::cam_P2 (BBa_J23115)

Bars (|) indicate differences between strains.


Plasmids

  • pCadCs (BBa_J23100_cadA_gltA_T, ColE1 origin, (T=Terminator (BBa_0010)))


Modifications
Gene Organism Modification
icd
Attenuation

Production
Metabolites Heterologous Production type Production Biomass Carbon source Time Scale Ref.
itaconate
Titer 2.27 g/L Glucose 49 h Flask [ 234 ]
itaconate
Substrate yield 0.767 mol/mol of substrate Glucose 49 h Flask [ 234 ]
itaconate
Titer 32.0 g/L Glucose Fed-Batch [ 234 ]
itaconate
Substrate yield 0.68 mol/mol of substrate Glucose Fed-Batch [ 234 ]
itaconate
Volumetric productivity 0.45 g/L/h Glucose Fed-Batch [ 234 ]

* Inferred from plots using RetroPlot.


References