Escherichia coli W3110 trpD9923/pTrc-ant3
Ancestors
Genotype with respect to wild type
F+ (λ) | λ- | inv(rrnD-rrnE) | F-, λ-, rph-1, IN (rrnD, rrnE) | trpD9923 | Plasmid pTrc-ant3 addedBars (|) indicate differences between strains.
Plasmids
-
pTrc-ant3 (Ptrc-antABC(P. aeruginosa PAO1))
Production
Metabolites | Heterologous | Production type | Production | Biomass | Carbon source | Time | Scale | Ref. |
---|---|---|---|---|---|---|---|---|
catechol |
|
Titer | 0.15 g/L | 1.69 g/L | Glucose | 74 h * | Flask | [ 236 ] |
anthranilate | Titer | 0.0 g/L | 1.69 g/L | Glucose | 74 h * | Flask | [ 236 ] | |
catechol |
|
Substrate yield | 0.05 g/g of sustrate | 1.69 g/L | Glucose | 74 h * | Flask | [ 236 ] |
catechol |
|
Specific production rate | 0.05 g/g DCW/h | 1.69 g/L | Glucose | 74 h * | Flask | [ 236 ] |
anthranilate | Specific production rate | 0.05 g/g DCW/h | 1.69 g/L | Glucose | 74 h * | Flask | [ 236 ] |
* Inferred from plots using RetroPlot.
References
- Balderas-Hernández, V.E., Treviño-Quintanilla, L.G., Hernández-Chávez, G. et al. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. Microb Cell Fact 13, 136 (2014). https://doi.org/10.1186/s12934-014-0136-x