Zhi‐Gang Qian, Xiao‐Xia Xia & Sang Yup Lee (2010). Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine. Biotechnology & Bioengineering.

Strains

• Escherichia coli XQ27
• Escherichia coli XQ56
• Escherichia coli XQ60
• Escherichia coli XQ64
• Escherichia coli XQ56 (p15CadA)
• Escherichia coli XQ27 (p15CadA)
• Escherichia coli XQ60 (p15CadA)
• Escherichia coli XQ64 (p15CadA)
• Escherichia coli WL3110 (p15CadA)
• Escherichia coli WL3110
• Escherichia coli W3110

Plasmids

• p15CadA
• pTac15K

Metabolite production

• cadaverine
  Titer
  • 9.61 g/L (Escherichia coli XQ56 (p15CadA) in Fed-Batch)
  • 1.31 g/L (Escherichia coli XQ56 (p15CadA) in Batch)
  • 1.19 g/L (Escherichia coli XQ27 (p15CadA) in Batch)
  • 1.31 g/L (Escherichia coli XQ64 (p15CadA) in Batch)
  • 1.31 g/L (Escherichia coli XQ60 (p15CadA) in Batch)
  • 0.79 g/L (Escherichia coli WL3110 (p15CadA) in Batch)
  Substrate yield
  • 0.12 g/g of sustrate (Escherichia coli XQ56 (p15CadA) in Fed-Batch)
  • 0.131 g/g of sustrate (Escherichia coli XQ56 (p15CadA) in Batch)
  • 0.119 g/g of sustrate (Escherichia coli XQ27 (p15CadA) in Batch)
  • 0.079 g/g of sustrate (Escherichia coli WL3110 (p15CadA) in Batch)
  Volumetric productivity
  • 0.32 g/L/h (Escherichia coli XQ56 (p15CadA) in Fed-Batch)