Tina Lütke-Eversloh & Gregory Stephanopoulos (2007). L-Tyrosine production by deregulated strains of Escherichia coli. Applied Microbiology and Biotechnology.

Strains

• Escherichia coli T2
• Escherichia coli K12 ΔtyrR
• Escherichia coli T1
• Escherichia coli K12 ΔtyrR (pCL1920::aroG^WT tyrA^WT)
• Escherichia coli K12 ΔtyrR (pCL1920::aroG^fbr tyrA^WT)
• Escherichia coli K12 ΔtyrR (pCL1920::aroG^WT tyrA^fbr)
• Escherichia coli K12 ΔtyrR (pCL1920::aroG^fbr tyrA^fbr)
• Escherichia coli K12 ΔtyrR (pCL1920::P_LtetO−1aroG^fbr tyrA^fbr ppsA)
• Escherichia coli K12 ΔtyrR (pCL1920::P_LtetO−1 aroG^fbr tyrA^fbr tktA)

Plasmids

• pCL1920
• pCL1920::aroG^WT tyrA^WT
• pCL1920::aroG^fbr tyrA^WT
• pCL1920::aroG^WT tyrA^fbr
• pCL1920::aroG^fbr tyrA^fbr
• pCL1920::P_LtetO−1aroG^fbr tyrA^fbr
• pCL1920::P_LtetO−1aroG^fbr tyrA^fbr ppsA
• pCL1920::P_LtetO−1 aroG^fbr tyrA^fbr tktA
• pCL1920::P_LtetO−1 aroG^fbr tyrA^fbr ppsA tktA

Metabolite production

• L-tyrosine
  Titer
  • 0.0 mg/L (Escherichia coli K12 ΔtyrR in Flask)
  • 346.0 mg/L (Escherichia coli T1 in Flask)
  • 3.8 g/L (Escherichia coli T1 in Fed-Batch)
  • 621.0 mg/L (Escherichia coli T2 in Flask)
  • 9.7 g/L (Escherichia coli T2 in Fed-Batch)
  • 6.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::aroG^WT tyrA^WT) in Flask)
  • 71.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::aroG^fbr tyrA^WT) in Flask)
  • 86.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::aroG^WT tyrA^fbr) in Flask)
  • 127.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::aroG^fbr tyrA^fbr) in Flask)
  • 466.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::P_LtetO−1aroG^fbr tyrA^fbr ppsA) in Flask)
  • 380.0 mg/L (Escherichia coli K12 ΔtyrR (pCL1920::P_LtetO−1 aroG^fbr tyrA^fbr tktA) in Flask)
  Substrate yield
  • 0.037 g/g of sustrate (Escherichia coli T1 in Fed-Batch)
  • 0.102 g/g of sustrate (Escherichia coli T2 in Fed-Batch)