Bo Xiong, Yongduo Zhu, Daoguang Tian, Shuai Jiang, Xiaoguang Fan, Qian Ma, Heyun Wu & Xixian Xie (2021). Flux redistribution of central carbon metabolism for efficient production of l-tryptophan in Escherichia coli. Biotechnology and bioengineering.

Strains

• Escherichia coli SX11
• Escherichia coli SX10
• Escherichia coli SX9
• Escherichia coli SX7
• Escherichia coli SX4
• Escherichia coli SX2
• Escherichia coli SX1
• Escherichia coli SX8
• Escherichia coli SX6
• Escherichia coli SX5
• Escherichia coli SX3

Metabolite production

• L-tryptophan
  Titer
  • 9.9 g/L (Escherichia coli SX1 in Flask)
  • 39.1 g/L (Escherichia coli SX1 in Fed-Batch)
  • 10.8 g/L (Escherichia coli SX2 in Flask)
  • 2.3 g/L (Escherichia coli SX3 in Flask)
  Substrate yield
  • 0.17 g/g of sustrate (Escherichia coli SX1 in Fed-Batch)
  • 0.13 g/g of sustrate (Escherichia coli SX1 in Flask)
  • 0.148 g/g of sustrate (Escherichia coli SX2 in Flask)