Lin Chen, Minliang Chen, Chengwei Ma & An-Ping Zeng (2018). Discovery of feed-forward regulation in L-tryptophan biosynthesis and its use in metabolic engineering of E. coli for efficient tryptophan bioproduction. Metabolic Engineering.

Strains

• Escherichia coli S092
• Escherichia coli S028
• Escherichia coli S028/pAnTrpC
• Escherichia coli S028/pEcTrpC
• Escherichia coli S028/pTAC242
• Escherichia coli S092/(pZATrpE40+pAnTrpC)
• Escherichia coli S092/pAnTrpC
• Escherichia coli S092/pScTrpC
• Escherichia coli S028/pTrc99A
• Escherichia coli S028TC/pTrpE(S40F)
• Escherichia coli S092/pEcTrpC
• Escherichia coli S028TC
• Escherichia coli S028/pTrpE(S40F)
• Escherichia coli S092/(pZATrpE40+pEcTrpC)

Plasmids

• pTrpE(S40F)
• pZATrpE40
• pTAC242
• pZS*24MCS
• pEcTrpC
• pScTrpC
• pAnTrpC
• pTrc99A

Metabolite production

• L-tryptophan
  Titer
  • 19.0 g/L (Escherichia coli S028 in Fed-Batch)
  • 575.0 mg/L (Escherichia coli S028TC/pTrpE(S40F) in Flask)
  • 2.12 g/L (Escherichia coli S092/pEcTrpC in Batch)
  • 3.13 g/L (Escherichia coli S092/pScTrpC in Batch)
  • 3.22 g/L (Escherichia coli S092/pAnTrpC in Batch)
  • 366.0 mg/L (Escherichia coli S028/pTrpE(S40F) in Flask)
  • 19.0 g/L (Escherichia coli S028/pTAC242 in Fed-Batch)
  • 19.0 g/L (Escherichia coli S028/pEcTrpC in Fed-Batch)
  • 29.0 g/L (Escherichia coli S028/pAnTrpC in Fed-Batch)
  Substrate yield
  • 0.15 g/g of sustrate (Escherichia coli S028 in Fed-Batch)
  • 0.07 g/g of sustrate (Escherichia coli S092/pEcTrpC in Batch)
  • 0.11 g/g of sustrate (Escherichia coli S092/pScTrpC in Batch)
  • 0.11 g/g of sustrate (Escherichia coli S092/pAnTrpC in Batch)
  • 0.14 g/g of sustrate (Escherichia coli S092/(pZATrpE40+pEcTrpC) in Fed-Batch)
  • 0.14 g/g of sustrate (Escherichia coli S028/pTAC242 in Fed-Batch)
  • 0.15 g/g of sustrate (Escherichia coli S028/pEcTrpC in Fed-Batch)
  • 0.17 g/g of sustrate (Escherichia coli S028/pAnTrpC in Fed-Batch)