Hemanshu Mundhada, Jose M. Seoane, Konstantin Schneider, Anna Koza, Hanne B. Christensen, Tobias Klein, Patrick V. Phaneuf, Markus Herrgard, Adam M. Feist & Alex T. Nielsen (2016). Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution. Metabolic Engineering.
Strains
- Escherichia coli Q1
- Escherichia coli ALE-3-2
- Escherichia coli ALE-4-2
- Escherichia coli ALE-5
- Escherichia coli ALE-3-2 (DE3)
- Escherichia coli ALE-4-2 (DE3)
- Escherichia coli ALE-5 (DE3)
- Escherichia coli Q1 (DE3)
- Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
- Escherichia coli Q1 (DE3) ThrA Y356C
- Escherichia coli Q1(DE3) ThrA S359R
- Escherichia coli Q1 (DE3) ThrA S357R
- Escherichia coli Q1(DE3) ThrA S357R Lrp D143G
- Escherichia coli Q1 (DE3) ThrA S357R Rho R87L
- Escherichia coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
- Escherichia coli ALE-3-2 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
- Escherichia coli ALE-4-2 (DE3) ( (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
- Escherichia coli ALE-5 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB)
- Escherichia coli MG1655
Metabolite production
-
(S)-2-hydroxyglutarate
-
Titer
- 12.1 g/L (Escherichia coli ALE-5 in Fed-Batch)
-
L-serine
-
Titer
- 3.7 g/L (Escherichia coli Q1 (DE3) (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) in Fed-Batch)
- 9.0 g/L (Escherichia coli Q1 (DE3) thrA S357R (pCDF-Duet-serAmut-serC, pACTCDuet-1-serB) in Fed-Batch)
- 37.3 g/L (Escherichia coli ALE-5 in Fed-Batch) Substrate yield
- 0.24 g/g of sustrate (Escherichia coli ALE-5 in Fed-Batch) Volumetric productivity
- 0.83 g/L/h (Escherichia coli ALE-5 in Fed-Batch)