Wang, C., Wu, J., Shi, B. et al. Improving l-serine formation by Escherichia coli by reduced uptake of produced l-serine. Microb Cell Fact 19, 66 (2020).
Strains
- Escherichia coli ES
- Escherichia coli ES-1
- Escherichia coli ES-12
- Escherichia coli ES-123
- Escherichia coli ES-1234
- Escherichia coli ES-1234/pSC-08
- Escherichia coli ES-124
- Escherichia coli ES-13
- Escherichia coli ES-134
- Escherichia coli ES-134/pSC-08
- Escherichia coli ES-13/pSC-08
- Escherichia coli ES-14
- Escherichia coli ES-1/pSC-08
- Escherichia coli ES-2
- Escherichia coli ES-3
- Escherichia coli ES-4
- Escherichia coli ES/pSC-08
- Escherichia coli W3110
Plasmids
Metabolite production
-
L-serine
-
Titer
- 384.0 mg/L (Escherichia coli ES-1234/pSC-08 in Flask)
- 26.3 g/L (Escherichia coli ES-1234/pSC-08 in Fed-Batch)
- 445.0 mg/L (Escherichia coli ES-134/pSC-08 in Flask)
- 34.8 g/L (Escherichia coli ES-134/pSC-08 in Fed-Batch)
- 385.0 mg/L (Escherichia coli ES-13/pSC-08 in Flask)
- 29.6 g/L (Escherichia coli ES-13/pSC-08 in Fed-Batch)
- 220.0 mg/L (Escherichia coli ES-1/pSC-08 in Flask)
- 23.8 g/L (Escherichia coli ES-1/pSC-08 in Fed-Batch)
- 129.0 mg/L (Escherichia coli ES/pSC-08 in Flask) Substrate yield
- 0.29 g/g of sustrate (Escherichia coli ES-1234/pSC-08 in Fed-Batch)
- 0.31 g/g of sustrate (Escherichia coli ES-13/pSC-08 in Fed-Batch)
- 0.25 g/g of sustrate (Escherichia coli ES-1/pSC-08 in Fed-Batch)
- 0.32 g/g of sustrate (Escherichia coli ES-134/pSC-08 in Fed-Batch)