Wenjuan Zha, Sheryl B. Rubin-Pitel, Zengyi Shao & Huimin Zhao (2009). Improving cellular malonyl-CoA level in Escherichia coli via metabolic engineering. Metabolic Engineering.

Strains

• Escherichia coli BWZ1
• Escherichia coli BWZ2
• Escherichia coli BWZ3
• Escherichia coli B-Acc
• Escherichia coli B-FabF
• Escherichia coli B-Acc/FabF
• Escherichia coli B-Acs/Acc
• Escherichia coli B-Acs/FabF
• Escherichia coli BWZ1-Acc
• Escherichia coli BWZ1-FabF
• Escherichia coli BWZ1-Acs/Acc
• Escherichia coli BWZ1-Acs/FabF
• Escherichia coli BWZ3-Acs/FabF
• Escherichia coli K12
• Escherichia coli BWZ3-Acs/Acc
• Escherichia coli BL21(DE3) (pACYC-PhlD)
• Escherichia coli B-Acc (pACYC-PhlD)
• Escherichia coli BL21(DE3)

Plasmids

• pACYC-FabF
• pACYC-Acs
• pACYC-FabF/Acs
• pACYC-PhlD
• pACYC-PhlD/Acs
• pRSF-Acc
• pACYCDuet-1
• pRSFDuet-1

Metabolite production

• acetyl-CoA
  Biomass-specific yield
  • 0.78 nmol/mg DCW (Escherichia coli B-Acc in Flask)
  • 0.72 nmol/mg DCW (Escherichia coli B-FabF in Flask)
  • 0.91 nmol/mg DCW (Escherichia coli B-Acc/FabF in Flask)
  • 0.34 nmol/mg DCW (Escherichia coli BWZ1-Acs/Acc in Flask)
  • 0.55 nmol/mg DCW (Escherichia coli BWZ1-Acc in Flask)
  • 0.81 nmol/mg DCW (Escherichia coli B-Acs/Acc in Flask)
  • 0.4 nmol/mg DCW (Escherichia coli BWZ3-Acs/Acc in Flask)
  • 0.8 nmol/mg DCW (Escherichia coli B-Acs/FabF in Flask)
  • 0.88 nmol/mg DCW (Escherichia coli BWZ1-FabF in Flask)
  • 0.81 nmol/mg DCW (Escherichia coli BWZ1-Acs/FabF in Flask)
  • 0.55 nmol/mg DCW (Escherichia coli BWZ1-Acs/FabF in Flask)
  • 0.93 nmol/mg DCW (Escherichia coli BL21(DE3) in Flask)
• malonyl-CoA
  Biomass-specific yield
  • 0.07 nmol/mg DCW (Escherichia coli BL21(DE3) in Flask)
  • 1.1 nmol/mg DCW (Escherichia coli BWZ3-Acs/Acc in Flask)
  • 0.45 nmol/mg DCW (Escherichia coli BWZ1-Acc in Flask)
  • 0.12 nmol/mg DCW (Escherichia coli BWZ1-FabF in Flask)
  • 1.06 nmol/mg DCW (Escherichia coli BWZ1-Acs/Acc in Flask)
  • 0.19 nmol/mg DCW (Escherichia coli BWZ1-Acs/FabF in Flask)
  • 0.45 nmol/mg DCW (Escherichia coli BWZ1-Acs/FabF in Flask)
  • 0.22 nmol/mg DCW (Escherichia coli B-Acc in Flask)
  • 0.28 nmol/mg DCW (Escherichia coli B-FabF in Flask)
  • 0.09 nmol/mg DCW (Escherichia coli B-Acc/FabF in Flask)
  • 0.19 nmol/mg DCW (Escherichia coli B-Acs/Acc in Flask)
  • 0.2 nmol/mg DCW (Escherichia coli B-Acs/FabF in Flask)
• phloroglucinol
  Titer
  • 1280.0 mg/L (Escherichia coli BWZ3-Acs/Acc (pACYC-PhlD) in Flask)
  • 1110.0 mg/L (Escherichia coli B-Acc (pACYC-PhlD) in Flask)
  • 510.0 mg/L (Escherichia coli BL21(DE3) (pACYC-PhlD) in Flask)