Zhi‐Gang Qian, Xiao‐Xia Xia & Sang Yup Lee (2009). Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine. Biotechnology & Bioengineering.

Strains

• Escherichia coli XQ26
• Escherichia coli XQ33
• Escherichia coli XQ38
• Escherichia coli XQ43
• Escherichia coli XQ47
• Escherichia coli XQ52
• Escherichia coli XQ26 (p15SpeC)
• Escherichia coli WL3110 (p15SpeC)
• Escherichia coli XQ26 (pKKSpeC)
• Escherichia coli XQ38 (p15SpeC)
• Escherichia coli XQ33 (p15SpeC)
• Escherichia coli XQ43 (p15SpeC)
• Escherichia coli XQ52 (p15SpeC)
• Escherichia coli WL3110
• Escherichia coli W3110

Plasmids

• pKK223-3
• p15SpeC
• pKKSpeC
• pTac15K

Metabolite production

• putrescine
  Titer
  • 18.0 g/L (Escherichia coli XQ43 in Fed-Batch)
  • 1.68 g/L (Escherichia coli XQ52 (p15SpeC) in Batch)
  • 24.2 g/L (Escherichia coli XQ52 (p15SpeC) in Fed-Batch)
  • 1.57 g/L (Escherichia coli XQ43 (p15SpeC) in Batch)
  • 21.7 g/L (Escherichia coli XQ43 (p15SpeC) in Fed-Batch)
  • 1.3 g/L (Escherichia coli XQ33 (p15SpeC) in Batch)
  • 0.84 g/L (Escherichia coli XQ38 (p15SpeC) in Batch)
  • 1.18 g/L (Escherichia coli XQ26 (p15SpeC) in Batch)
  • 0.09 g/L (Escherichia coli XQ26 (pKKSpeC) in Batch)
  • 0.25 g/L (Escherichia coli WL3110 (p15SpeC) in Batch)
  Volumetric productivity
  • 0.63 g/L/h (Escherichia coli XQ43 in Fed-Batch)
  • 0.75 g/L/h (Escherichia coli XQ52 (p15SpeC) in Fed-Batch)