Heyun Wu, Yanjun Li, Qian Ma, Qiang Li, Zifan Jia, Bo Yang, Qingyang Xu, Xiaoguang Fan, Chenglin Zhang, Ning Chen & Xixian Xie (2018). Metabolic engineering of Escherichia coli for high-yield uridine production. Metabolic Engineering.

Strains

• Escherichia coli UR2-1
• Escherichia coli UR2-2
• Escherichia coli UR2-3
• Escherichia coli UR2-4
• Escherichia coli UR2-5
• Escherichia coli UR3-1
• Escherichia coli UR3-2
• Escherichia coli UR3-3
• Escherichia coli UR4
• Escherichia coli UR5-1
• Escherichia coli UR5-2
• Escherichia coli UR6
• Escherichia coli UR1
• Escherichia coli DH5α
• Escherichia coli W3110

Metabolite production

• uracil
  Titer
  • 4.5 g/L (Escherichia coli UR1 in Flask)
  • 0.62 g/L (Escherichia coli UR2-5 in Flask)
  • 0.75 g/L (Escherichia coli UR3-1 in Flask)
  • 0.67 g/L (Escherichia coli UR3-2 in Flask)
  • 0.92 g/L (Escherichia coli UR3-3 in Flask)
  • 1.03 g/L (Escherichia coli UR4 in Flask)
  • 1.12 g/L (Escherichia coli UR5-1 in Flask)
  • 1.21 g/L (Escherichia coli UR5-2 in Flask)
  • 0.52 g/L (Escherichia coli UR6 in Flask)
  • 0.9 g/L (Escherichia coli UR6 in Fed-Batch)
• uridine
  Titer
  • 0.0 g/L (Escherichia coli W3110 in Flask)
  • 5.5 g/L (Escherichia coli UR1 in Flask)
  • 3.3 g/L (Escherichia coli UR1 in Flask)
  • 7.8 g/L (Escherichia coli UR2-5 in Flask)
  • 9.3 g/L (Escherichia coli UR3-1 in Flask)
  • 10.3 g/L (Escherichia coli UR3-3 in Flask)
  • 12.3 g/L (Escherichia coli UR4 in Flask)
  • 12.8 g/L (Escherichia coli UR5-1 in Flask)
  • 13.8 g/L (Escherichia coli UR5-2 in Flask)
  • 14.5 g/L (Escherichia coli UR6 in Flask)
  • 70.3 g/L (Escherichia coli UR6 in Fed-Batch)
  • 8.2 g/L (Escherichia coli UR3-2 in Flask)
  Substrate yield
  • 0.057 g/g of sustrate (Escherichia coli UR2-5 in Flask)
  • 0.073 g/g of sustrate (Escherichia coli UR3-1 in Flask)
  • 0.086 g/g of sustrate (Escherichia coli UR3-3 in Flask)
  • 0.107 g/g of sustrate (Escherichia coli UR4 in Flask)
  • 0.116 g/g of sustrate (Escherichia coli UR5-1 in Flask)
  • 0.157 g/g of sustrate (Escherichia coli UR5-2 in Flask)
  • 0.161 g/g of sustrate (Escherichia coli UR6 in Flask)
  • 0.259 g/g of sustrate (Escherichia coli UR6 in Fed-Batch)
  • 0.062 g/g of sustrate (Escherichia coli UR3-2 in Flask)
  Volumetric productivity
  • 1.1 g/L/h (Escherichia coli UR6 in Fed-Batch)