Heyun Wu, Daoguang Tian, Xiaoguang Fan, Weiming Fan, Yue Zhang, Shuai Jiang, Chenhui Wen, Qian Ma, Ning Chen & Xixian Xie (2020). Highly Efficient Production of l‑Histidine from Glucose by Metabolically Engineered Escherichia coli. ACS Synthetic Biology.
Strains
- Escherichia coli HW1
- Escherichia coli HW1-1
- Escherichia coli HW1-2
- Escherichia coli HW1-3
- Escherichia coli HW1-4
- Escherichia coli HW1-5
- Escherichia coli HW2-1
- Escherichia coli HW2-2
- Escherichia coli HW2-3
- Escherichia coli HW2-4
- Escherichia coli HW3-1
- Escherichia coli HW3-2
- Escherichia coli HW3-3
- Escherichia coli HW3-4
- Escherichia coli HW3-5
- Escherichia coli HW4-1
- Escherichia coli HW4-2
- Escherichia coli HW4-3
- Escherichia coli HW4-4
- Escherichia coli HW4-5
- Escherichia coli HW5-1
- Escherichia coli HW6-1
- Escherichia coli HW6-2
- Escherichia coli HW6-3
- Escherichia coli HW6-4
- Escherichia coli W3110
Metabolite production
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L-histidine
-
Titer
- 0.08 g/L (Escherichia coli HW1 in Flask)
- 0.12 g/L (Escherichia coli HW1-1 in Flask)
- 0.24 g/L (Escherichia coli HW1-2 in Flask)
- 0.83 g/L (Escherichia coli HW1-5 in Flask)
- 1.3 g/L (Escherichia coli HW2-1 in Flask)
- 1.4 g/L (Escherichia coli HW2-2 in Flask)
- 3.8 g/L (Escherichia coli HW2-4 in Flask)
- 4.2 g/L (Escherichia coli HW3-1 in Flask)
- 4.0 g/L (Escherichia coli HW3-2 in Flask)
- 4.6 g/L (Escherichia coli HW3-3 in Flask)
- 4.9 g/L (Escherichia coli HW3-4 in Flask)
- 4.9 g/L (Escherichia coli HW3-5 in Flask)
- 5.4 g/L (Escherichia coli HW4-1 in Flask)
- 5.6 g/L (Escherichia coli HW4-2 in Flask)
- 7.3 g/L (Escherichia coli HW4-4 in Flask)
- 8.2 g/L (Escherichia coli HW4-5 in Flask)
- 10.1 g/L (Escherichia coli HW5-1 in Flask)
- 11.8 g/L (Escherichia coli HW6-3 in Flask)
- 66.5 g/L (Escherichia coli HW6-3 in Fed-Batch)
- 10.1 g/L (Escherichia coli HW6-4 in Flask)
- 2.8 g/L (Escherichia coli HW2-3 in Flask) Volumetric productivity
- 1.5 g/L/h (Escherichia coli HW6-3 in Fed-Batch) Substrate yield
- 0.23 g/g of sustrate (Escherichia coli HW6-3 in Fed-Batch)