Jian Wang, Ruihua Zhang, Yan Zhang, Yaping Yang, Yuheng Lin & Yajun Yan (2019). Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production. Metabolic Engineering.

Strains

• Escherichia coli ATCC 31884
• Escherichia coli JW2
• Escherichia coli JW1
• Escherichia coli JW3
• Escherichia coli JW4
• Escherichia coli JW5
• Escherichia coli JW6
• Escherichia coli JW7
• Escherichia coli JW8
• Escherichia coli AA1
• Escherichia coli AA2
• Escherichia coli AA3
• Escherichia coli AA4
• Escherichia coli TP1
• Escherichia coli TP2
• Escherichia coli TP3
• Escherichia coli MA2
• Escherichia coli MA3
• Escherichia coli MA5
• Escherichia coli MA1
• Escherichia coli MA4
• Escherichia coli QH4
• Escherichia coli XL1-Blue

Plasmids

• F'
• pCS27
• pCS-trpE^fbrG
• pCS-trpE^fbrG-APTA
• pCS-serA*
• pCS-serA*-psrAwt
• pCS-serA*-prsA-A95T
• pCS-serA*-prsA-G226V
• pCS-trpE^fbrtrpG-serA*-prsA-A95T
• pSA74
• pSA-trpDBCA
• pZE12-luc
• pZE-PAPC

Metabolite production

• anthranilate
  Titer
  • 0.85 g/L (Escherichia coli AA1 in Flask)
  • 1.78 g/L (Escherichia coli AA4 in Flask)
  • 0.84 g/L (Escherichia coli QH4 in Flask)
  • 1.0 g/L (Escherichia coli JW2 in Flask)
  • 1.78 g/L (Escherichia coli JW3 in Flask)
  • 0.56 g/L (Escherichia coli QH4 in Flask)
  • 1.35 g/L (Escherichia coli JW2 in Flask)
  • 1.9 g/L (Escherichia coli JW3 in Flask)
  • 0.0 g/L (Escherichia coli TP1 in Flask)
  • 0.56 g/L (Escherichia coli AA1 in Flask)
  • 1.38 g/L (Escherichia coli AA3 in Flask)
  • 1.9 g/L (Escherichia coli AA4 in Flask)
  • 0.37 g/L (Escherichia coli MA2 in Flask)
  • 0.97 g/L (Escherichia coli MA3 in Flask)
  Substrate yield
  • 0.143 g/g of sustrate (Escherichia coli AA4 in Flask)
  • 0.077 g/g of sustrate (Escherichia coli AA3 in Flask)
  • 0.054 g/g of sustrate (Escherichia coli AA1 in Flask)
  • 0.054 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.077 g/g of sustrate (Escherichia coli JW2 in Flask)
  • 0.143 g/g of sustrate (Escherichia coli JW3 in Flask)
  • 0.046 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.131 g/g of sustrate (Escherichia coli JW2 in Flask)
  • 0.179 g/g of sustrate (Escherichia coli JW3 in Flask)
  • 0.178 g/g of sustrate (Escherichia coli AA4 in Flask)
  • 0.131 g/g of sustrate (Escherichia coli AA3 in Flask)
  • 0.046 g/g of sustrate (Escherichia coli AA1 in Flask)
• L-tryptophan
  Titer
  • 0.73 g/L (Escherichia coli QH4 in Flask)
  • 1.73 g/L (Escherichia coli JW7 in Flask)
  • 1.32 g/L (Escherichia coli JW8 in Flask)
  • 0.82 g/L (Escherichia coli QH4 in Flask)
  • 1.31 g/L (Escherichia coli JW7 in Flask)
  • 0.97 g/L (Escherichia coli JW8 in Flask)
  • 0.73 g/L (Escherichia coli TP1 in Flask)
  • 1.73 g/L (Escherichia coli TP2 in Flask)
  • 1.31 g/L (Escherichia coli TP2 in Flask)
  • 0.97 g/L (Escherichia coli TP3 in Flask)
  • 0.82 g/L (Escherichia coli TP1 in Flask)
  Substrate yield
  • 0.037 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.168 g/g of sustrate (Escherichia coli JW8 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.09 g/g of sustrate (Escherichia coli JW7 in Flask)
  • 0.132 g/g of sustrate (Escherichia coli JW7 in Flask)
  • 0.08 g/g of sustrate (Escherichia coli JW8 in Flask)
  • 0.132 g/g of sustrate (Escherichia coli TP2 in Flask)
  • 0.038 g/g of sustrate (Escherichia coli TP1 in Flask)
  • 0.168 g/g of sustrate (Escherichia coli TP3 in Flask)
• catechol
  Titer
  • 0.14 g/L (Escherichia coli MA2 in Flask)
  • 0.8 g/L (Escherichia coli MA2 in Flask)
  • 0.43 g/L (Escherichia coli MA3 in Flask)
  • 0.43 g/L (Escherichia coli MA3 in Flask)
• cis,cis-muconate
  Titer
  • 1.6 g/L (Escherichia coli MA3 in Flask)
  • 0.47 g/L (Escherichia coli MA3 in Flask)
  • 0.68 g/L (Escherichia coli MA1 in Flask)
  • 0.46 g/L (Escherichia coli MA2 in Flask)
  • 0.89 g/L (Escherichia coli MA1 in Flask)
  • 0.86 g/L (Escherichia coli MA4 in Flask)
  • 0.71 g/L (Escherichia coli MA5 in Flask)
  • 1.82 g/L (Escherichia coli MA2 in Flask)
  • 0.47 g/L (Escherichia coli JW3 in Flask)
  • 0.46 g/L (Escherichia coli JW2 in Flask)
  • 0.68 g/L (Escherichia coli QH4 in Flask)
  • 1.6 g/L (Escherichia coli JW3 in Flask)
  • 1.82 g/L (Escherichia coli JW2 in Flask)
  • 0.89 g/L (Escherichia coli QH4 in Flask)
  Substrate yield
  • 0.029 g/g of sustrate (Escherichia coli JW3 in Flask)
  • 0.028 g/g of sustrate (Escherichia coli JW2 in Flask)
  • 0.059 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.125 g/g of sustrate (Escherichia coli JW3 in Flask)
  • 0.071 g/g of sustrate (Escherichia coli QH4 in Flask)
  • 0.122 g/g of sustrate (Escherichia coli JW2 in Flask)