Soo Yun Moon, Soon Ho Hong, Tae Yong Kim & Sang Yup Lee (2008). Metabolic engineering of Escherichia coli for the production of malic acid. Biochemical Engineering Journal.

Strains

• Escherichia coli WGS-3
• Escherichia coli WGS-10/p104ManPck
• Escherichia coli WGS-10
• Escherichia coli W3110/p10499A
• Escherichia coli W3110/p104ColiPck
• Escherichia coli W3110/p104ManPck
• Escherichia coli WGS-3/p10499A
• Escherichia coli WGS-3/p104ColiPck
• Escherichia coli WGS-3/p104ManPck
• Escherichia coli WGS-10/p10499A
• Escherichia coli WGS-10/p104ColiPck
• Escherichia coli XL1-Blue
• Escherichia coli W3110

Plasmids

• pUC19
• p10499A
• p104ColiPck
• p104ManPck

Metabolite production

• (S)-malate
  Titer
  • 0.0 g/L (Escherichia coli WGS-3/p10499A in Batch)
  • 0.0 g/L (Escherichia coli WGS-3/p104ColiPck in Batch)
  • 9.62 g/L (Escherichia coli WGS-3/p104ManPck in Batch)
  • 9.25 g/L (Escherichia coli WGS-10/p104ManPck in Batch)
  • 0.0 g/L (Escherichia coli WGS-10/p10499A in Batch)
  • 1.42 g/L (Escherichia coli WGS-10/p104ColiPck in Batch)
  • 0.0 g/L (Escherichia coli W3110/p10499A in Batch)
  • 0.0 g/L (Escherichia coli W3110/p104ColiPck in Batch)
  • 0.0 g/L (Escherichia coli W3110/p104ManPck in Batch)
  Substrate yield
  • 0.0 mol/mol of substrate (Escherichia coli WGS-3/p10499A in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli WGS-3/p104ColiPck in Batch)
  • 0.61 mol/mol of substrate (Escherichia coli WGS-3/p104ManPck in Batch)
  • 0.56 mol/mol of substrate (Escherichia coli WGS-10/p104ManPck in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli WGS-10/p10499A in Batch)
  • 0.11 mol/mol of substrate (Escherichia coli WGS-10/p104ColiPck in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli W3110/p10499A in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli W3110/p104ColiPck in Batch)
  • 0.0 mol/mol of substrate (Escherichia coli W3110/p104ManPck in Batch)
  Volumetric productivity
  • 0.0 g/L/h (Escherichia coli WGS-3/p10499A in Batch)
  • 0.0 g/L/h (Escherichia coli WGS-3/p104ColiPck in Batch)
  • 0.69 g/L/h (Escherichia coli WGS-3/p104ManPck in Batch)
  • 0.74 g/L/h (Escherichia coli WGS-10/p104ManPck in Batch)
  • 0.0 g/L/h (Escherichia coli WGS-10/p10499A in Batch)
  • 0.09 g/L/h (Escherichia coli WGS-10/p104ColiPck in Batch)
  • 0.0 g/L/h (Escherichia coli W3110/p10499A in Batch)
  • 0.0 g/L/h (Escherichia coli W3110/p104ColiPck in Batch)
  • 0.0 g/L/h (Escherichia coli W3110/p104ManPck in Batch)