Gen Nonaka & Kazuhiro Takumi (2017). Cysteine degradation gene yhaM, encoding cysteine desulfidase, serves as a genetic engineering target to improve cysteine production in Escherichia coli. AMB Express.

Strains

• Escherichia coli MG1655 (pMIV-cysE5, pACYC177)
• Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1)
• Escherichia coli MG1655 (pMIV-cysE5)
• Escherichia coli MG1655 ΔyhaM
• Escherichia coli MG1655 ΔyhaM (pMIV-cysE5)

Plasmids

• pSTV29
• pSTV-ydeD5
• pSTV-yhaM7
• pACYC177
• pACYC-yhaM1
• pMIV-cysE5

Metabolite production

• L-cysteine
  Titer
  • 74.6 mg/L (Escherichia coli MG1655 (pMIV-cysE5, pACYC177) in Flask)
  • 14.9 mg/L (Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1) in Flask)
  • 316.2 mg/L (Escherichia coli MG1655 ΔyhaM (pMIV-cysE5) in Flask)
  • 64.6 mg/L (Escherichia coli MG1655 (pMIV-cysE5) in Flask)
  Substrate yield
  • 0.187 % (Escherichia coli MG1655 (pMIV-cysE5, pACYC177) in Flask)
  • 0.037 % (Escherichia coli MG1655 (pMIV-cysE5, pACYC-yhaM1) in Flask)
  • 0.81 % (Escherichia coli MG1655 ΔyhaM (pMIV-cysE5) in Flask)
  • 0.164 % (Escherichia coli MG1655 (pMIV-cysE5) in Flask)