Balderas-Hernández, V.E., Treviño-Quintanilla, L.G., Hernández-Chávez, G. et al. Catechol biosynthesis from glucose in Escherichia coli anthranilate-overproducer strains by heterologous expression of anthranilate 1,2-dioxygenase from Pseudomonas aeruginosa PAO1. Microb Cell Fact 13, 136 (2014). https://doi.org/10.1186/s12934-014-0136-x

Strains

• Escherichia coli W3110 trpD9923
• Escherichia coli W3110 trpD9923 PTS^-
• Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3
• Escherichia coli W3110 trpD9923/pTrc-ant3
• Escherichia coli trpD9923 PTS^-/pTrc-ant3

Plasmids

• pJLBaroG^fbrtktA
• pTrc-ant3

Metabolite production

• catechol
  Titer
  • 0.37 g/L (Escherichia coli trpD9923 PTS^-/pTrc-ant3 in Flask)
  • 0.15 g/L (Escherichia coli W3110 trpD9923/pTrc-ant3 in Flask)
  • 0.41 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Flask)
  • 4.47 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 2.51 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 2.81 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 1.83 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  Substrate yield
  • 0.09 g/g of sustrate (Escherichia coli trpD9923 PTS^-/pTrc-ant3 in Flask)
  • 0.05 g/g of sustrate (Escherichia coli W3110 trpD9923/pTrc-ant3 in Flask)
  • 0.1 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Flask)
  • 16.0 % (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.07 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.08 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.06 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  Biomass-specific productivity
  • 0.03 mg/g DCW/h (Escherichia coli trpD9923 PTS^-/pTrc-ant3 in Flask)
  Specific production rate
  • 0.05 g/g DCW/h (Escherichia coli W3110 trpD9923/pTrc-ant3 in Flask)
  • 0.07 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Flask)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.01 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
• anthranilate
  Titer
  • 0.22 g/L (Escherichia coli trpD9923 PTS^-/pTrc-ant3 in Flask)
  • 0.0 g/L (Escherichia coli W3110 trpD9923/pTrc-ant3 in Flask)
  • 0.15 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Flask)
  • 8.18 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 2.12 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 8.61 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 6.28 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 2.89 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 2.21 g/L (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  Substrate yield
  • 0.09 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.17 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.09 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.18 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.11 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.1 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.09 g/g of sustrate (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  Specific production rate
  • 0.03 g/g DCW/h (Escherichia coli trpD9923 PTS^-/pTrc-ant3 in Flask)
  • 0.05 g/g DCW/h (Escherichia coli W3110 trpD9923/pTrc-ant3 in Flask)
  • 0.07 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Flask)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.07 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.01 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA/pTrc-ant3 in Fed-Batch)
  • 0.01 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)
  • 0.02 g/g DCW/h (Escherichia coli W3110 trpD9923/pJLBaroG^fbrtktA in Fed-Batch)