Pu, W., Chen, J., Zhou, Y. et al. Systems metabolic engineering of Escherichia coli for hyper-production of 5‑aminolevulinic acid. Biotechnol Biofuels 16, 31 (2023).

Strains

• Escherichia coli ZLEcA4
• Escherichia coli ALA1
• Escherichia coli ALA2
• Escherichia coli ALA3
• Escherichia coli ALA4
• Escherichia coli ALA5
• Escherichia coli ALA6
• Escherichia coli ALA7
• Escherichia coli ALA8
• Escherichia coli ALA10
• Escherichia coli ALA11
• Escherichia coli ALA12
• Escherichia coli ALA13
• Escherichia coli ALA14
• Escherichia coli ALA15
• Escherichia coli ALA16
• Escherichia coli ALA17
• Escherichia coli ALA18
• Escherichia coli ALA19
• Escherichia coli ALA20
• Escherichia coli ALA21
• Escherichia coli ALA22
• Escherichia coli ALA23
• Escherichia coli ALA24
• Escherichia coli ALA25
• Escherichia coli ALA26
• Escherichia coli ALA9
• Escherichia coli MG1655

Plasmids

• pWSK29
• pEASY-Blunt
• pSB4C5
• pACYC184
• pUC57-sRNA
• pZPA17
• pZPA18
• pKD46
• pZPA43
• pZCA9
• pZCA9p
• pTrc99A-hemA
• pET21a–hemA
• pZSA13
• pZGA24
• pZSA14
• pZSA41
• pZCA135
• pZCA137
• pZCA136
• pZPW70
• pZPW71
• pZPW72
• pZPW73
• pZPW74
• pZPW76
• pZPW77
• pZPW78
• pZPW79
• pZPW80
• pZPW81
• pZPW82
• pZPW83
• pZPW84
• pZPW85
• pZPW86
• pZPW87
• pZCA134
• pTrc99A

Metabolite production

• 5-aminolevulinate
  Titer
  • 1.225 g/L (Escherichia coli ALA1 in Flask)
  • 1.358 g/L (Escherichia coli ALA2 in Flask)
  • 1.408 g/L (Escherichia coli ALA3 in Flask)
  • 1.358 g/L (Escherichia coli ALA4 in Flask)
  • 1.383 g/L (Escherichia coli ALA5 in Flask)
  • 1.4 g/L (Escherichia coli ALA6 in Flask)
  • 1.508 g/L (Escherichia coli ALA7 in Flask)
  • 1.56 g/L (Escherichia coli ALA8 in Flask)
  • 1.83 g/L (Escherichia coli ALA8 in Flask)
  • 2.44 g/L (Escherichia coli ALA9 in Flask)
  • 4.05 g/L (Escherichia coli ALA10 in Flask)
  • 4.91 g/L (Escherichia coli ALA11 in Flask)
  • 5.69 g/L (Escherichia coli ALA13 in Flask)
  • 5.39 g/L (Escherichia coli ALA12 in Flask)
  • 6.857 g/L (Escherichia coli ALA13 in Flask)
  • 8.32 g/L (Escherichia coli ALA14 in Flask)
  • 7.048 g/L (Escherichia coli ALA15 in Flask)
  • 6.857 g/L (Escherichia coli ALA16 in Flask)
  • 3.905 g/L (Escherichia coli ALA17 in Flask)
  • 6.19 g/L (Escherichia coli ALA18 in Flask)
  • 7.72 g/L (Escherichia coli ALA19 in Flask)
  • 7.83 g/L (Escherichia coli ALA20 in Flask)
  • 3.143 g/L (Escherichia coli ALA21 in Flask)
  • 7.89 g/L (Escherichia coli ALA22 in Flask)
  • 8.539 g/L (Escherichia coli ALA24 in Flask)
  • 6.834 g/L (Escherichia coli ALA25 in Flask)
  • 8.008 g/L (Escherichia coli ALA13 in Flask)
  • 8.235 g/L (Escherichia coli ALA23 in Flask)
  • 9.79 g/L (Escherichia coli ALA14 in Flask)
  • 8.539 g/L (Escherichia coli ALA24 in Flask)
  • 6.834 g/L (Escherichia coli ALA25 in Flask)
  • 30.7 g/L (Escherichia coli ALA26 in Fed-Batch)
  • 4.5 g/L (Escherichia coli ALA14 in Fed-Batch)
  Substrate yield
  • 0.205 mol/mol of substrate (Escherichia coli ALA1 in Flask)
  • 0.221 mol/mol of substrate (Escherichia coli ALA2 in Flask)
  • 0.235 mol/mol of substrate (Escherichia coli ALA3 in Flask)
  • 0.223 mol/mol of substrate (Escherichia coli ALA4 in Flask)
  • 0.221 mol/mol of substrate (Escherichia coli ALA5 in Flask)
  • 0.228 mol/mol of substrate (Escherichia coli ALA6 in Flask)
  • 0.258 mol/mol of substrate (Escherichia coli ALA7 in Flask)
  • 0.292 mol/mol of substrate (Escherichia coli ALA8 in Flask)
  • 0.312 mol/mol of substrate (Escherichia coli ALA8 in Flask)
  • 0.364 mol/mol of substrate (Escherichia coli ALA9 in Flask)
  • 0.364 mol/mol of substrate (Escherichia coli ALA10 in Flask)
  • 0.43 mol/mol of substrate (Escherichia coli ALA11 in Flask)
  • 0.495 mol/mol of substrate (Escherichia coli ALA13 in Flask)
  • 0.429 mol/mol of substrate (Escherichia coli ALA12 in Flask)
  • 0.45 mol/mol of substrate (Escherichia coli ALA13 in Flask)
  • 0.559 mol/mol of substrate (Escherichia coli ALA14 in Flask)
  • 0.445 mol/mol of substrate (Escherichia coli ALA15 in Flask)
  • 0.436 mol/mol of substrate (Escherichia coli ALA16 in Flask)
  • 0.319 mol/mol of substrate (Escherichia coli ALA17 in Flask)
  • 0.628 mol/mol of substrate (Escherichia coli ALA18 in Flask)
  • 0.535 mol/mol of substrate (Escherichia coli ALA19 in Flask)
  • 0.574 mol/mol of substrate (Escherichia coli ALA20 in Flask)
  • 0.277 mol/mol of substrate (Escherichia coli ALA21 in Flask)
  • 0.517 mol/mol of substrate (Escherichia coli ALA22 in Flask)
  • 0.532 mol/mol of substrate (Escherichia coli ALA26 in Fed-Batch)
  Volumetric productivity
  • 1.02 g/L/h (Escherichia coli ALA26 in Fed-Batch)