Jiang, Youming; Zheng, Tianwen; Ye, Xiaohan; Xin, Fengxue; Zhang, Wenming; Dong, Weiliang; Ma, Jiangfeng & Jiang, Min. Metabolic engineering of Escherichia coli for l-malate production anaerobically. Microbial Cell Factories. 2020, 19(1).

Strains

• Escherichia coli BA002
• Escherichia coli BA040
• Escherichia coli BA041
• Escherichia coli BA042
• Escherichia coli BA050
• Escherichia coli BA051
• Escherichia coli BA052
• Escherichia coli BA060
• Escherichia coli BA061
• Escherichia coli BA062
• Escherichia coli BA063

Plasmids

• pTrc99A
• pTrc99a-mdh
• pTrc99a-pncB
• pTrc99a-ppc
• pTrc99a-pyc
• pTrc99a-Bspck
• pTrc99a-Bspck-mdh
• pTrc99a-Bspck-pncB

Metabolite production

• (S)-malate
  Titer
  • 13.14 g/L (Escherichia coli BA063 in Flask)
  • 1.73 g/L (Escherichia coli BA040 in Flask)
  • 3.53 g/L (Escherichia coli BA041 in Flask)
  • 4.39 g/L (Escherichia coli BA042 in Flask)
  • 3.22 g/L (Escherichia coli BA050 in Flask)
  • 5.3 g/L (Escherichia coli BA051 in Flask)
  • 4.39 g/L (Escherichia coli BA052 in Flask)
  • 9.39 g/L (Escherichia coli BA061 in Flask)
  • 9.19 g/L (Escherichia coli BA062 in Flask)
  • 28.5 g/L (Escherichia coli BA063 in Fed-Batch)
  Substrate yield
  • 0.73 g/g of sustrate (Escherichia coli BA063 in Flask)
  • 0.2 g/g of sustrate (Escherichia coli BA040 in Flask)
  • 0.34 g/g of sustrate (Escherichia coli BA041 in Flask)
  • 0.3 g/g of sustrate (Escherichia coli BA042 in Flask)
  • 0.46 g/g of sustrate (Escherichia coli BA051 in Flask)
  • 0.63 g/g of sustrate (Escherichia coli BA061 in Flask)
  • 0.61 g/g of sustrate (Escherichia coli BA062 in Flask)
  • 0.69 g/g of sustrate (Escherichia coli BA063 in Fed-Batch)
  • 0.34 g/g of sustrate (Escherichia coli BA050 in Flask)
  • 0.44 g/g of sustrate (Escherichia coli BA052 in Flask)
• pyruvate
  Titer
  • 0.97 g/L (Escherichia coli BA040 in Flask)
  • 1.85 g/L (Escherichia coli BA041 in Flask)
  • 2.52 g/L (Escherichia coli BA042 in Flask)
  • 1.81 g/L (Escherichia coli BA050 in Flask)
  • 1.69 g/L (Escherichia coli BA051 in Flask)
  • 0.58 g/L (Escherichia coli BA052 in Flask)
  • 2.87 g/L (Escherichia coli BA061 in Flask)
  • 2.89 g/L (Escherichia coli BA062 in Flask)
  • 2.67 g/L (Escherichia coli BA063 in Flask)
• succinate
  Titer
  • 1.23 g/L (Escherichia coli BA040 in Flask)
  • 2.46 g/L (Escherichia coli BA041 in Flask)
  • 1.87 g/L (Escherichia coli BA042 in Flask)
  • 1.58 g/L (Escherichia coli BA050 in Flask)
  • 1.46 g/L (Escherichia coli BA051 in Flask)
  • 1.85 g/L (Escherichia coli BA052 in Flask)
  • 1.79 g/L (Escherichia coli BA061 in Flask)
  • 1.45 g/L (Escherichia coli BA062 in Flask)
  • 1.25 g/L (Escherichia coli BA063 in Flask)